In this video:
Session by Mallikarjun Reddy, Associate Director – Quality, Clearsynth
Topic: Dealing with challenges in testing and use of Stable Isotopic Labelled Standards
+ Labeled standards are preferred as Internal standards (ISTD) because they improve efficiency of bioassay or quantification method
+ The phytochemical properties of Label standards are almost similar to their unlabeled standards.
+ Deuterated and other isotope labelled internal standard have similar extraction recovery, ionisation response and matrix effect which improves the accuracy of quantification method
+ ISTD also reduces chromatography time, making an assay more robust by increasing the throughput and lowering rejection rates
+ Degree of labeling is very important while selecting an isotopic standard