Modern instrumental quantitative analysis by HPTLC is popular these days. The products have the following characteristic features: They are visual chromatogram, simple, multiple sample handling in parallel, low analysis and maintenance costs, negligible sample clean – up, derivatisation, hyphenation with MS etc.
However, the glass chamber with manual handling, a carry – over from TLC, resulted in the development of Achille’s heel. This is now a history with the availability of Camag, Switzerland, which has developed automatic development chamber – 2. It is a completely ‘closed’ development chamber.
Automated Development Chamber ADC-2 from Camag, Switzerland retains the 20 x 10 cm twin trough glass chamber for development. This eliminates absolutely genius design eliminating the need to revalidate older methods. Moreover, the ADC-2 enables a control over the humidity content of the layer ensuring universal reproducibility of chromatographic conditions. The new regulatory HPTLC SOP ( effective August 2015) is optimally performed with help of this instrument.
The SOP for using ADC – 2 is extremely simple.
The plate (with samples applied) in ADC- 2 needs to be hanged and pre-loaded method recalled followed by pouring the mobile for saturation and for development and ultimately pressing the start button. After the audio signal, chromatographed and dried plate should be removed.
HPTLC today is fully automated but in individual steps. Sample application as bands and evaluation by scanner or image visualiser are fully automated and well accepted to HPTLC. The ADC- 2 adds cGLP compliance to HPTLC.
Anchrom Enterprises (I)
101, ‘A’ Wing, Aniket Apartment,
Navghar Road, Mulund (East),
Mumbai – 400 081, India.
Tel.: 091 22 21639928 – 31/7506792122
Fax: 091 22 21639927
E-mail: [email protected]